Human NOS3/eNOS (Nitric Oxide Synthase 3, Endothelial) CLIA Kit
The Human NOS3 (eNOS/Nitric Oxide Synthase 3 - Endothelial) CLIA Kit is a cutting-edge tool designed for the precise measurement of NOS3 levels in human samples including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research purposes.NOS3, also known as eNOS, is a key enzyme involved in the production of nitric oxide, which plays a vital role in cardiovascular health by regulating blood vessel tone and blood flow.
Dysregulation of NOS3 activity has been linked to various diseases including hypertension, atherosclerosis, and diabetes, highlighting its significance as a potential therapeutic target.By quantifying NOS3 levels, researchers can gain valuable insights into the molecular mechanisms underlying cardiovascular diseases and explore new approaches for treatment and prevention. The Human NOS3 CLIA Kit is a valuable asset for studies focusing on NOS3 biology and its implications in health and disease.
Product Name:
Human NOS3/eNOS (Nitric Oxide Synthase 3, Endothelial) CLIA Kit
SKU:
HUES00474
Target:
Human NOS3/eNOS (Nitric Oxide Synthase 3, Endothelial)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human NOS3/eNOS. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human NOS3/eNOS and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human NOS3/eNOS, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human NOS3/eNOS. You can calculate the concentration of Human NOS3/eNOS in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-102
101-113
91-102
Average (%)
96
107
96
1:4
Range (%)
89-103
102-115
87-99
Average (%)
97
108
94
1:8
Range (%)
99-116
100-116
85-95
Average (%)
106
107
90
1:16
Range (%)
96-112
91-103
97-109
Average (%)
104
98
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-102
97
EDTA plasma (n=5)
98-116
106
Cell culture media (n=5)
90-105
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
49.99
142.23
366.47
45.51
148.76
366.5
Standard deviation
6.46
14.22
22.32
5.18
13.92
29.72
C V (%)
12.92
10.0
6.09
11.38
9.36
8.11
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human NOS3/eNOS concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human NOS3/eNOS in samples. No significant cross-reactivity or interference between Human NOS3/eNOS and analogues was observed.
