The Human NPYR5 (Neuropeptide Y Receptor Y5) CLIA Kit is specifically designed for the quantitative detection of NPYR5 levels in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides accurate and reliable results, making it essential for various research applications.Neuropeptide Y receptor Y5 is a key protein involved in mediating the effects of neuropeptide Y, a neurotransmitter with diverse physiological functions. Dysregulation of this receptor has been linked to various disorders such as obesity, anxiety, and cardiovascular diseases, making it a valuable target for therapeutic interventions.
By accurately measuring NPYR5 levels, researchers can gain valuable insights into the role of this receptor in health and disease, paving the way for the development of novel treatment strategies. The Human NPYR5 CLIA Kit is a powerful tool for studying the intricate mechanisms underlying these conditions and advancing our understanding of neuropeptide Y signaling pathways.
Product Name:
Human NPYR5 (Neuropeptide Y Receptor Y5) CLIA Kit
SKU:
HUES00506
Target:
Human NPYR5 (Neuropeptide Y Receptor Y5)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human NPYR5. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human NPYR5 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human NPYR5, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human NPYR5. You can calculate the concentration of Human NPYR5 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
102-117
101-117
93-104
Average (%)
108
109
98
1:4
Range (%)
89-101
99-115
99-112
Average (%)
94
106
106
1:8
Range (%)
93-111
96-108
90-105
Average (%)
101
102
97
1:16
Range (%)
86-99
92-104
97-110
Average (%)
93
99
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-101
94
EDTA plasma (n=5)
95-107
101
Cell culture media (n=5)
95-109
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
193.54
413.64
1608.52
178.59
392.06
1689.06
Standard deviation
20.07
46.87
142.03
20.9
43.36
131.41
C V (%)
10.37
11.33
8.83
11.7
11.06
7.78
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human NPYR5 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human NPYR5 in samples. No significant cross-reactivity or interference between Human NPYR5 and analogues was observed.
