The Human NRP1 (Neuropilin-1) CLIA Kit is specifically designed for the accurate quantification of Neuropilin-1 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research purposes.Neuropilin-1 is a critical receptor protein involved in cell signaling and plays a key role in various physiological and pathological processes, including angiogenesis, axon guidance, and immune response modulation.
Dysregulation of Neuropilin-1 has been linked to a range of diseases, including cancer, autoimmune disorders, and cardiovascular conditions, highlighting its importance as a biomarker for disease detection and therapeutic development.With its reliable performance and broad applicability in different research areas, the Human NRP1 CLIA Kit is an indispensable tool for scientists and researchers looking to investigate the roles of Neuropilin-1 in health and disease.
Product Name:
Human NRP1 (Neuropilin 1) CLIA Kit
SKU:
HUES00709
Target:
Human NRP1 (Neuropilin 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human NRP1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human NRP1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human NRP1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human NRP1. You can calculate the concentration of Human NRP1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-99
92-104
97-110
Average (%)
93
97
102
1:4
Range (%)
92-104
99-115
97-107
Average (%)
98
107
102
1:8
Range (%)
85-98
94-110
84-97
Average (%)
91
102
91
1:16
Range (%)
97-110
86-98
89-103
Average (%)
104
91
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-109
103
EDTA plasma (n=5)
101-117
107
Cell culture media (n=5)
98-115
106
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
51.58
146.97
353.82
56.3
134.77
355.49
Standard deviation
5.17
14.46
39.52
5.64
15.85
39.64
C V (%)
10.02
9.84
11.17
10.02
11.76
11.15
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human NRP1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human NRP1 in samples. No significant cross-reactivity or interference between Human NRP1 and analogues was observed.
