The Human NTN1 (Netrin-1) CLIA Kit is specifically formulated for the precise measurement of Netrin-1 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for various research purposes.Netrin-1 is a key protein that plays a vital role in guiding axon growth and influencing cell migration during development. Dysregulation of Netrin-1 has been linked to various diseases and disorders, including cancer, developmental disorders, and neurological conditions.
As such, monitoring Netrin-1 levels can provide valuable insights into disease progression and potential therapeutic interventions.Overall, the Human NTN1 (Netrin-1) CLIA Kit is a valuable tool for researchers studying the role of Netrin-1 in health and disease, offering reliable and robust performance for a wide range of applications.
Product Name:
Human Ntn1 (Netrin 1) CLIA Kit
SKU:
HUES01205
Target:
Human Ntn1 (Netrin 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human Ntn1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human Ntn1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human Ntn1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human Ntn1. You can calculate the concentration of Human Ntn1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
101-115
104-121
89-102
Average (%)
109
110
96
1:4
Range (%)
97-113
99-111
90-102
Average (%)
105
105
95
1:8
Range (%)
89-104
84-96
87-102
Average (%)
95
90
93
1:16
Range (%)
91-104
97-114
93-105
Average (%)
96
105
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-112
105
EDTA plasma (n=5)
85-100
92
Cell culture media (n=5)
101-117
107
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
40.47
109.15
394.69
36.77
98.45
393.42
Standard deviation
3.29
10.66
44.13
3.9
10.8
42.76
C V (%)
8.13
9.77
11.18
10.61
10.97
10.87
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human Ntn1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human Ntn1 in samples. No significant cross-reactivity or interference between Human Ntn1 and analogues was observed.
