The Human OCLN (Occludin) CLIA Kit is a cutting-edge assay designed for the quantitative measurement of occludin levels in human samples, including serum, plasma, and cell culture supernatants. This highly sensitive and specific kit ensures accurate and reproducible results, making it an invaluable tool for a variety of research applications.Occludin is a critical protein involved in maintaining the integrity of tight junctions, which are essential for controlling the passage of molecules across cell barriers.
Dysregulation of occludin has been implicated in various diseases, including inflammatory bowel disease, celiac disease, and multiple sclerosis, highlighting its significance as a potential biomarker for studying and treating these conditions.With its advanced technology and user-friendly format, the Human OCLN CLIA Kit is an essential tool for researchers investigating the role of occludin in health and disease, offering valuable insights into the underlying mechanisms of various pathological conditions.
Product Name:
Human OCLN (Occludin) CLIA Kit
SKU:
HUES00631
Target:
Human OCLN (Occludin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human OCLN. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human OCLN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human OCLN, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human OCLN. You can calculate the concentration of Human OCLN in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
101-119
97-112
91-101
Average (%)
109
103
96
1:4
Range (%)
103-119
98-114
88-99
Average (%)
109
106
93
1:8
Range (%)
97-113
85-99
89-102
Average (%)
104
90
95
1:16
Range (%)
99-117
85-99
90-104
Average (%)
107
91
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-108
101
EDTA plasma (n=5)
90-105
96
Cell culture media (n=5)
99-112
104
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
109.15
195.21
975.23
117.29
191.99
954.49
Standard deviation
13.07
22.16
104.84
11.71
22.31
87.81
C V (%)
11.97
11.35
10.75
9.98
11.62
9.2
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human OCLN concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human OCLN in samples. No significant cross-reactivity or interference between Human OCLN and analogues was observed.
