The Human OCLN (Occludin) ELISA Kit is specifically designed for the precise measurement of occludin levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for various research purposes.Occludin is a key protein involved in maintaining the integrity of epithelial and endothelial cell barriers, playing a crucial role in regulating the permeability of these barriers. Dysregulation of occludin has been linked to various diseases, including inflammatory bowel disease, chronic inflammation, and autoimmune disorders, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions. Overall, the Human OCLN (Occludin) ELISA Kit provides a reliable and efficient tool for researchers looking to investigate the role of occludin in health and disease, opening up new possibilities for advancing our understanding and treatment of relevant medical conditions.
Product Name:
Human OCLN (Occludin) ELISA Kit
SKU:
HUES02170
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.1 ng/mL
Detection range:
0.16-10 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
