The Human Osteonectin ELISA Kit (HUES02397) is an advanced and reliable tool for the detection of osteonectin levels in human samples such as serum, plasma, and cell culture supernatants. Osteonectin, also known as SPARC (Secreted Protein Acidic and Rich in Cysteine), is a key extracellular matrix protein involved in bone development, wound healing, and tissue remodeling.This ELISA kit offers high sensitivity and specificity, ensuring accurate and reproducible results for a wide range of research applications. Osteonectin is a critical factor in various pathological conditions, including osteoporosis, cancer metastasis, and fibrosis, making it a valuable biomarker for studying these diseases and developing potential therapeutic interventions.
With easy-to-follow protocols and rapid assay procedures, the Human Osteonectin ELISA Kit provides researchers with a powerful tool to investigate the role of osteonectin in health and disease. Order now to accelerate your research and gain valuable insights into the biology of osteonectin.
Product Name:
Human ON (Osteonectin) ELISA Kit
SKU:
HUES02397
Target:
Human ON (Osteonectin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ON. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ON and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ON, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human ON. You can calculate the concentration of Human ON in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-99
90-103
95-109
Average (%)
94
97
102
1:4
Range (%)
92-108
85-99
84-93
Average (%)
99
92
89
1:8
Range (%)
91-107
87-100
85-97
Average (%)
98
93
90
1:16
Range (%)
89-103
84-97
85-101
Average (%)
96
89
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-101
96
EDTA plasma (n=5)
88-102
93
Cell culture media (n=5)
89-104
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.08
1.91
8.12
1.14
1.95
7.33
Standard deviation
0.07
0.11
0.38
0.06
0.1
0.37
C V (%)
6.48
5.76
4.68
5.26
5.13
5.05
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human ON concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ON in samples. No significant cross-reactivity or interference between Human ON and analogues was observed.
