The Human OPN (Osteopontin) ELISA Kit is specifically designed for the precise measurement of osteopontin levels in human samples including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for various research applications.Osteopontin is a key protein involved in bone metabolism, immune system regulation, and inflammation.
It plays a crucial role in various diseases such as cancer, cardiovascular diseases, and autoimmune disorders, making it a valuable biomarker for research and therapeutic development.With its high-quality components and user-friendly protocol, the Human OPN ELISA Kit from Assay Genie is a reliable tool for scientists and researchers studying osteopontin and its impact on health and disease.
Product Name:
Human OPN (Osteopontin) ELISA Kit
SKU:
HUES02393
Target:
Human OPN (Osteopontin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human OPN. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human OPN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human OPN, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human OPN. You can calculate the concentration of Human OPN in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-111
87-102
87-99
Average (%)
102
94
92
1:4
Range (%)
91-108
81-94
87-101
Average (%)
99
88
92
1:8
Range (%)
86-98
85-96
85-97
Average (%)
93
90
92
1:16
Range (%)
94-106
84-95
86-99
Average (%)
99
89
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-100
95
EDTA plasma (n=5)
97-111
102
Cell culture media (n=5)
85-95
90
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.97
2.46
7.02
1.05
2.26
6.75
Standard deviation
0.06
0.14
0.28
0.05
0.11
0.23
C V (%)
6.19
5.69
3.99
4.76
4.87
3.41
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human OPN concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human OPN in samples. No significant cross-reactivity or interference between Human OPN and analogues was observed.
