Human OxLDL (Oxidized Low Density Lipoprotein) CLIA Kit
The Human OXLDL (Oxidized Low-Density Lipoprotein) CLIA Kit is a cutting-edge tool for the precise measurement of oxidized LDL levels in human biological samples. This kit offers exceptional accuracy and specificity, guaranteeing consistent and dependable results for various research purposes.Oxidized LDL is a key biomarker associated with atherosclerosis, inflammation, and oxidative stress, playing a critical role in the development of cardiovascular diseases.
By accurately measuring OXLDL levels, researchers can gain valuable insights into disease progression, risk assessment, and potential therapeutic interventions.With its advanced technology and reliable performance, the Human OXLDL CLIA Kit is an indispensable resource for studying cardiovascular health, lipid metabolism, and oxidative damage. Invest in this innovative kit today and unlock new possibilities in your research endeavors.
Product Name:
Human OxLDL (Oxidized Low Density Lipoprotein) CLIA Kit
SKU:
HUES00115
Target:
Human OxLDL (Oxidized Low Density Lipoprotein)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human OxLDL. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human OxLDL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human OxLDL, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human OxLDL. You can calculate the concentration of Human OxLDL in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-102
88-102
94-107
Average (%)
94
95
101
1:4
Range (%)
91-103
101-118
88-104
Average (%)
97
109
95
1:8
Range (%)
100-115
97-109
89-101
Average (%)
106
103
94
1:16
Range (%)
87-101
86-96
91-105
Average (%)
92
91
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-112
106
EDTA plasma (n=5)
84-100
91
Cell culture media (n=5)
99-113
105
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
46.66
114.62
419.97
44.9
106.96
439.64
Standard deviation
3.87
12.68
42.08
5.04
12.84
47.13
C V (%)
8.29
11.06
10.02
11.22
12.0
10.72
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human OxLDL concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human OxLDL in samples. No significant cross-reactivity or interference between Human OxLDL and analogues was observed.
