Human OxLDL (Oxidized Low Density Lipoprotein) ELISA Kit
The Oxidized Low-Density Lipoprotein (OxLDL) ELISA Kit is expertly crafted to quantitatively measure OxLDL levels in various human samples. OxLDL, a crucial biomarker of oxidative stress and a key player in atherosclerosis development, serves as an essential indicator of lipid peroxidation and vascular damage. Accurate quantification of OxLDL provides valuable insights into cardiovascular health, allowing for a deeper understanding of lipid metabolism and cardiovascular risk assessment. By ensuring exceptional sensitivity and specificity, this ELISA kit delivers precise and reproducible results. Manufactured under rigorous quality control standards, the kit offers robust performance and user-friendly procedures, making it an ideal choice for both research and clinical applications. Rely on Assay Genie's OxLDL ELISA Kit for accurate and reliable quantification of this pivotal cardiovascular biomarker in your studies.
Product Name:
Human OxLDL (Oxidized Low Density Lipoprotein) ELISA Kit
SKU:
AEES00057
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
37.5 pg/mL
Detection range:
62.5-4000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
