The Human OGG1 (Oxoguanine Glycosylase 1) ELISA Kit is specially designed for the precise measurement of OGG1 levels in human serum, plasma, and cell culture supernatants. This kit is known for its exceptional sensitivity and specificity, ensuring accurate and consistent results, which makes it perfect for a variety of research purposes.OGG1 is a vital enzyme involved in DNA repair, specifically in the removal of 8-oxoguanine, a common form of DNA damage caused by oxidative stress.
Dysregulation of OGG1 has been linked to various diseases, including cancer, neurodegenerative disorders, and aging. Therefore, OGG1 serves as a critical biomarker for studying these conditions and potential therapeutic interventions.Don't miss out on the opportunity to advance your research with the Human OGG1 ELISA Kit. Get yours today and unlock new insights into DNA repair mechanisms and disease pathology.
Product Name:
Human Oxoguanine Glycosylase 1 (OGG1) ELISA Kit
Product Code:
HUDL02134
Size:
96 Assays
Target:
OGG1
Synonyms:
HMMH, HOGG1, MUTM, OGH1, 8-Oxoguanine DNA Glycosylase, DNA-(apurinic or apyrimidinic site) lyase, N-glycosylase/DNA lyase
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
