Human P3CP (Procollagen 3 C-Terminal ProPeptide) CLIA Kit
The Human P3CP (Procollagen 3 C-terminal propeptide) CLIA Kit is a powerful tool for the precise measurement of P3CP levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers superior sensitivity and specificity, ensuring accurate and reproducible results that are essential for various research applications.P3CP is an important marker for collagen synthesis and extracellular matrix remodeling. It plays a key role in conditions like fibrosis, connective tissue disorders, and wound healing, making it a valuable biomarker for studying these pathological processes and developing innovative therapeutic strategies.
The Human P3CP CLIA Kit from Assay Genie provides researchers with a reliable and efficient solution for analyzing P3CP levels in biological samples, ultimately helping to advance our understanding and treatment of related diseases and disorders.
Product Name:
Human P3CP (Procollagen 3 C-Terminal ProPeptide) CLIA Kit
SKU:
HUES00512
Target:
Human P3CP (Procollagen 3 C-Terminal ProPeptide)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human Pâ…¢CP. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human Pâ…¢CP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human Pâ…¢CP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human Pâ…¢CP. You can calculate the concentration of Human Pâ…¢CP in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-98
86-98
86-100
Average (%)
93
92
92
1:4
Range (%)
100-115
100-115
95-109
Average (%)
105
107
101
1:8
Range (%)
91-105
88-101
98-109
Average (%)
97
95
103
1:16
Range (%)
97-113
92-107
86-99
Average (%)
105
99
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-102
97
EDTA plasma (n=5)
94-107
100
Cell culture media (n=5)
87-101
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
37.86
84.52
354.03
39.28
80.0
362.67
Standard deviation
3.91
6.19
32.04
4.91
6.82
25.93
C V (%)
10.33
7.32
9.05
12.5
8.53
7.15
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human Pâ…¢CP concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human Pâ…¢CP in samples. No significant cross-reactivity or interference between Human Pâ…¢CP and analogues was observed.
