Human PACAP-38 (Pituitary Adenylate Cyclase Activating Polypeptide 38) ELISA Kit
The Human PACAP-38 (Pituitary Adenylate Cyclase Activating Polypeptide-38) ELISA Kit is specially designed for the precise quantification of PACAP-38 levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications.PACAP-38 is a critical neuropeptide involved in various physiological processes, including neuronal survival, development, and neurotransmitter regulation.
Dysregulation of PACAP-38 has been linked to neurological disorders, inflammatory conditions, and metabolic diseases, making it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions.Overall, the Human PACAP-38 ELISA Kit provides researchers with a reliable tool for investigating the role of PACAP-38 in health and disease, offering valuable insights into its molecular mechanisms and potential clinical implications.
Product Name:
Human PACAP-38 (Pituitary Adenylate Cyclase Activating Polypeptide 38) ELISA Kit
SKU:
HUES03307
Target:
Human PACAP-38 (Pituitary Adenylate Cyclase Activating Polypeptide 38)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PACAP-38. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PACAP-38 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PACAP-38, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human PACAP-38. You can calculate the concentration of Human PACAP-38 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-103
90-105
95-109
Average (%)
94
96
101
1:4
Range (%)
87-99
84-96
83-93
Average (%)
94
89
88
1:8
Range (%)
91-107
83-97
86-97
Average (%)
98
88
92
1:16
Range (%)
89-102
85-98
83-96
Average (%)
95
90
89
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-106
100
EDTA plasma (n=5)
86-100
91
Cell culture media (n=5)
85-96
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.02
3.21
9.84
1.01
3.33
10.01
Standard deviation
0.07
0.19
0.42
0.05
0.16
0.4
C V (%)
6.86
5.92
4.27
4.95
4.8
4.0
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human PACAP-38 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PACAP-38 in samples. No significant cross-reactivity or interference between Human PACAP-38 and analogues was observed.
