Human PADI4 (Peptidyl Arginine Deiminase Type IV) CLIA Kit
The Human PADI4 (Peptidyl Arginine Deiminase Type IV) CLIA Kit is a high-quality assay designed for the precise measurement of PADI4 levels in human serum, plasma, and cell culture supernatants. With exceptional sensitivity and specificity, this kit delivers accurate and consistent results, making it an invaluable tool for various research applications.PADI4 is an important enzyme involved in the post-translational modification of proteins, specifically the conversion of arginine residues to citrulline. Dysregulation of PADI4 has been linked to various autoimmune diseases, including rheumatoid arthritis and lupus, as well as cancer and other inflammatory conditions.
Understanding the role of PADI4 in these diseases is crucial for developing targeted therapies and diagnostic markers.With the Human PADI4 CLIA Kit, researchers can confidently quantify PADI4 levels in biological samples, enabling a deeper insight into its biological functions and potential therapeutic implications. Trust in the accuracy and reliability of this kit for your research needs.
Product Name:
Human PADI4 (Peptidyl Arginine Deiminase Type IV) CLIA Kit
SKU:
HUES00765
Target:
Human PADI4 (Peptidyl Arginine Deiminase Type IV)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human PADI4. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PADI4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PADI4, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human PADI4. You can calculate the concentration of Human PADI4 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-108
100-112
102-116
Average (%)
101
107
108
1:4
Range (%)
96-110
90-103
90-104
Average (%)
102
97
98
1:8
Range (%)
91-106
101-115
98-111
Average (%)
97
107
104
1:16
Range (%)
101-117
100-116
90-102
Average (%)
108
107
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
98-110
103
EDTA plasma (n=5)
99-114
106
Cell culture media (n=5)
88-100
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
196.88
406.01
1941.91
213.15
388.35
1806.5
Standard deviation
21.26
39.75
205.84
27.41
35.34
134.04
C V (%)
10.8
9.79
10.6
12.86
9.1
7.42
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human PADI4 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PADI4 in samples. No significant cross-reactivity or interference between Human PADI4 and analogues was observed.
