Human PAI1 (Plasminogen Activator Inhibitor 1) CLIA Kit
The Human PAI-1 (Plasminogen Activator Inhibitor-1) CLIA Kit is a highly sensitive and specific assay designed for the precise measurement of PAI-1 levels in human samples, including serum, plasma, and cell culture supernatants. This kit provides accurate and reliable results, making it ideal for a variety of research applications.PAI-1 is a key regulator of the fibrinolytic system, playing a crucial role in maintaining the balance between blood clot formation and dissolution. Dysregulation of PAI-1 levels has been implicated in a range of pathological conditions, including thrombosis, cardiovascular diseases, and inflammatory disorders.
By measuring PAI-1 levels, researchers can gain valuable insights into the underlying mechanisms of these diseases and identify potential therapeutic targets.Overall, the Human PAI-1 CLIA Kit offers a powerful tool for studying PAI-1 biology and its role in disease pathology, with the potential to drive advancements in both research and clinical practice.
Product Name:
Human PAI1 (Plasminogen Activator Inhibitor 1) CLIA Kit
SKU:
HUES01122
Target:
Human PAI1 (Plasminogen Activator Inhibitor 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human PAI1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PAI1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PAI1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human PAI1. You can calculate the concentration of Human PAI1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-104
90-104
84-99
Average (%)
98
96
90
1:4
Range (%)
101-115
87-101
95-107
Average (%)
109
92
101
1:8
Range (%)
88-99
92-104
92-109
Average (%)
94
98
100
1:16
Range (%)
84-99
97-113
101-117
Average (%)
91
104
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-111
103
EDTA plasma (n=5)
98-111
106
Cell culture media (n=5)
98-110
104
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
110.61
294.29
935.72
120.92
316.11
857.54
Standard deviation
12.6
34.79
104.71
13.94
28.67
92.36
C V (%)
11.39
11.82
11.19
11.53
9.07
10.77
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human PAI1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PAI1 in samples. No significant cross-reactivity or interference between Human PAI1 and analogues was observed.
