Human PAI1 (Plasminogen Activator Inhibitor 1) ELISA Kit
The Human PAI-1 (Plasminogen Activator Inhibitor-1) ELISA Kit is a reliable tool for the precise measurement of PAI-1 levels in human biological samples including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit guarantees accurate and reproducible results, making it suitable for a variety of research applications.PAI-1 is a key regulator of fibrinolysis, playing a critical role in blood clotting and thrombosis.
Dysregulation of PAI-1 has been linked to various diseases including cardiovascular disorders, obesity, and diabetes, highlighting its importance as a biomarker for studying these conditions and exploring potential therapeutic interventions.Get your Human PAI-1 ELISA Kit today and unlock new insights into the role of PAI-1 in health and disease.
Product Name:
Human PAI1 (Plasminogen Activator Inhibitor 1) ELISA Kit
SKU:
HUES03013
Target:
Human PAI1 (Plasminogen Activator Inhibitor 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PAI1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PAI1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PAI1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human PAI1. You can calculate the concentration of Human PAI1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-97
97-111
94-106
Average (%)
92
103
100
1:4
Range (%)
89-104
80-94
82-97
Average (%)
95
86
88
1:8
Range (%)
91-106
84-97
82-94
Average (%)
97
89
88
1:16
Range (%)
90-102
80-94
88-102
Average (%)
95
86
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-104
95
EDTA plasma (n=5)
86-100
93
Cell culture media (n=5)
86-100
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.55
1.44
4.92
0.56
1.5
4.68
Standard deviation
0.03
0.08
0.27
0.03
0.09
0.2
C V (%)
5.45
5.56
5.49
5.36
6.0
4.27
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human PAI1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PAI1 in samples. No significant cross-reactivity or interference between Human PAI1 and analogues was observed.
