Human Pancreas Specific Transcription Factor 1a (PTF1a) ELISA Kit
The Human Pancreas-Specific Transcription Factor 1a (PTF1A) ELISA Kit is a cutting-edge tool designed for the precise measurement of PTF1A levels in human samples such as serum, plasma, and cell culture supernatants. With its superior sensitivity and specificity, this kit delivers accurate and consistent results, making it an invaluable resource for a variety of research purposes.PTF1A is a key transcription factor that plays a crucial role in pancreatic development and function. Dysregulation of PTF1A has been linked to various pancreatic disorders, including pancreatic cancer and diabetes, making it a valuable marker for the study of these conditions and the development of potential therapeutic interventions.
Overall, the Human PTF1A ELISA Kit offers researchers a powerful tool to investigate the role of PTF1A in pancreatic biology and disease, ultimately leading to a better understanding of these conditions and the potential for novel treatment strategies.
Product Name:
Human Pancreas Specific Transcription Factor 1a (PTF1a) ELISA Kit
Product Code:
HUDL02442
Size:
96 Assays
Target:
PTF1a
Synonyms:
PTF1-A, PTF1-p48, Class A basic helix-loop-helix protein 29, bHLH transcription factor p48, p48 DNA-binding subunit of transcription factor PTF1
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-103
96
Heparin plasma (n=5)
89-102
95
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
