Human PCDHgammaA2 (Protocadherin Gamma A2) CLIA Kit
The Human PCDHgammaA2 (Protocadherin gamma-A2) CLIA Kit is a powerful tool for the precise measurement of PCDHgammaA2 levels in human samples including serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit delivers accurate and dependable results, making it ideal for various research applications.PCDHgammaA2, a member of the protocadherin family, is known for its role in cell adhesion and neuronal development.
Dysregulation of PCDHgammaA2 has been linked to various diseases such as neurological disorders and cancer, highlighting its significance as a biomarker for disease detection and therapy development.By utilizing the Human PCDHgammaA2 CLIA Kit, researchers can gain valuable insights into the role of PCDHgammaA2 in disease pathology and progression, ultimately paving the way for innovative diagnostic and therapeutic strategies.
Product Name:
Human PCDHgammaA2 (Protocadherin Gamma A2) CLIA Kit
SKU:
HUES00584
Target:
Human PCDHgammaA2 (Protocadherin Gamma A2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human PCDHγA2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PCDHγA2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PCDHγA2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human PCDHγA2. You can calculate the concentration of Human PCDHγA2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-100
92-103
87-102
Average (%)
93
97
94
1:4
Range (%)
92-105
101-117
103-115
Average (%)
97
109
109
1:8
Range (%)
93-109
91-105
98-115
Average (%)
100
96
105
1:16
Range (%)
101-117
100-114
91-106
Average (%)
109
108
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-103
98
EDTA plasma (n=5)
90-106
97
Cell culture media (n=5)
86-97
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
99.31
277.5
823.08
91.9
251.77
822.9
Standard deviation
9.37
28.83
53.17
9.3
27.34
58.1
C V (%)
9.44
10.39
6.46
10.12
10.86
7.06
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human PCDHγA2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PCDHγA2 in samples. No significant cross-reactivity or interference between Human PCDHγA2 and analogues was observed.
