The Human PCT (Procalcitonin) CLIA Kit is a cutting-edge diagnostic tool designed for the accurate measurement of procalcitonin levels in human samples such as serum, plasma, and cell culture supernatants. This highly sensitive and specific kit guarantees precise and trustworthy results, making it an invaluable resource for various research endeavors.Procalcitonin is a critical biomarker that serves as an indicator of bacterial infections and sepsis in the body.
By measuring procalcitonin levels, healthcare professionals can quickly assess the severity of an infection and determine the appropriate course of treatment. This kit is instrumental in studying infectious diseases, monitoring patient progress, and developing targeted therapies to combat bacterial infections effectively.
Product Name:
Human PCT (Procalcitonin) CLIA Kit
SKU:
HUES00848
Target:
Human PCT (Procalcitonin)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human PCT. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PCT and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PCT, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human PCT. You can calculate the concentration of Human PCT in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-108
101-114
93-104
Average (%)
100
108
99
1:4
Range (%)
95-110
86-102
104-117
Average (%)
103
93
110
1:8
Range (%)
100-117
102-120
98-111
Average (%)
107
110
105
1:16
Range (%)
92-107
98-114
86-99
Average (%)
99
104
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
84-96
90
EDTA plasma (n=5)
92-105
99
Cell culture media (n=5)
92-107
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
40.81
102.27
357.64
39.58
100.61
326.98
Standard deviation
3.27
8.75
23.28
5.09
11.98
22.73
C V (%)
8.01
8.56
6.51
12.86
11.91
6.95
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human PCT concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PCT in samples. No significant cross-reactivity or interference between Human PCT and analogues was observed.
