The Human PF4 (Platelet Factor 4) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of PF4 levels in human serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it ideal for a variety of research applications.PF4 is a critical protein involved in blood clotting and inflammation, playing a key role in conditions such as heparin-induced thrombocytopenia and cardiovascular diseases.
Studying PF4 levels can provide valuable insights into these conditions and aid in the development of potential therapies.Order the Human PF4 CLIA Kit today to advance your research and uncover new discoveries in the field of platelet biology and coagulation disorders.
Product Name:
Human PF4 (Platelet Factor 4) CLIA Kit
SKU:
HUES01165
Target:
Human PF4 (Platelet Factor 4)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human PF4. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PF4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PF4, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human PF4. You can calculate the concentration of Human PF4 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-102
93-110
100-117
Average (%)
95
100
107
1:4
Range (%)
94-108
103-116
87-99
Average (%)
101
110
93
1:8
Range (%)
104-119
92-106
88-101
Average (%)
110
98
95
1:16
Range (%)
96-111
87-99
102-114
Average (%)
103
93
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
100-116
106
EDTA plasma (n=5)
98-116
106
Cell culture media (n=5)
88-102
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.93
2.17
8.85
0.9
2.17
8.1
Standard deviation
0.09
0.18
0.78
0.09
0.17
0.69
C V (%)
9.68
8.29
8.81
10.0
7.83
8.52
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human PF4 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PF4 in samples. No significant cross-reactivity or interference between Human PF4 and analogues was observed.
