The Human Placental Growth Factor (PGF) CLIA Kit offered by Assay Genie is a cutting-edge tool for detecting PGF levels in human samples with precision and accuracy. This kit is specifically designed for assessing PGF in serum, plasma, and cell culture supernatants, providing researchers with reliable and reproducible results for a variety of applications.PGF is a key growth factor involved in angiogenesis, the process of forming new blood vessels. Its role in promoting vascular growth and cell proliferation makes it a crucial biomarker for studying diseases such as cancer, cardiovascular disorders, and neurodegenerative conditions.
By using the Human PGF CLIA Kit, researchers can gain valuable insights into the role of PGF in disease progression and potential therapeutic interventions.Trust Assay Genie for high-quality, dependable assays that enable groundbreaking research in the field of angiogenesis and vascular biology. Upgrade your experiments with the Human PGF CLIA Kit and uncover new discoveries in the realm of growth factor biology.
Product Name:
Human PGF (Placental Growth Factor) CLIA Kit
SKU:
HUES00882
Target:
Human PGF (Placental Growth Factor)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human PGF. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PGF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PGF, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human PGF. You can calculate the concentration of Human PGF in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-98
94-109
86-100
Average (%)
92
102
91
1:4
Range (%)
89-104
89-103
93-107
Average (%)
95
94
100
1:8
Range (%)
97-113
91-103
98-115
Average (%)
103
97
106
1:16
Range (%)
98-113
86-98
89-101
Average (%)
104
91
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-113
105
EDTA plasma (n=5)
93-105
99
Cell culture media (n=5)
93-107
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
23.77
64.76
206.1
23.62
69.23
208.67
Standard deviation
2.4
6.77
22.4
2.51
4.99
18.55
C V (%)
10.1
10.45
10.87
10.63
7.21
8.89
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human PGF concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PGF in samples. No significant cross-reactivity or interference between Human PGF and analogues was observed.
