The Human PGF (Placental Growth Factor) ELISA Kit is specifically designed for the precise measurement of placental growth factor levels in human samples such as serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit delivers accurate and consistent results, making it an invaluable tool for various research applications.PGF, also known as placental growth factor, is a key protein that plays a crucial role in angiogenesis and vascular development.
Its involvement in conditions like cancer, cardiovascular diseases, and pregnancy-related complications highlight its importance as a biomarker for studying and potentially treating these conditions.With the Human PGF ELISA Kit, researchers can uncover valuable insights into PGF levels and its impact on various physiological processes, paving the way for advancements in diagnostics and personalized medicine.
Product Name:
Human PGF (Placental Growth Factor) ELISA Kit
SKU:
HUES02569
Target:
Human PGF (Placental Growth Factor)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PGF. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PGF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PGF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human PGF. You can calculate the concentration of Human PGF in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-113
85-98
88-101
Average (%)
105
90
94
1:4
Range (%)
95-112
80-91
90-104
Average (%)
103
87
96
1:8
Range (%)
96-111
86-100
86-99
Average (%)
104
91
91
1:16
Range (%)
98-114
82-95
89-100
Average (%)
105
89
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-102
94
EDTA plasma (n=5)
90-101
96
Cell culture media (n=5)
87-100
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
6.05
13.47
58.46
5.75
12.85
55.66
Standard deviation
0.35
0.72
2.66
0.38
0.53
2.26
C V (%)
5.74
5.34
4.55
6.65
4.13
4.06
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human PGF concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PGF in samples. No significant cross-reactivity or interference between Human PGF and analogues was observed.
