The Human PK2 (Prokineticin-2) CLIA Kit is a highly sensitive and accurate assay designed for the detection of prokineticin-2 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional specificity, ensuring reliable and reproducible results for a variety of research applications.Prokineticin-2 is a key protein involved in various physiological processes, including regulation of circadian rhythms, neurogenesis, and pain perception. Dysregulation of prokineticin-2 has been linked to conditions such as inflammatory diseases, sleep disorders, and cancer, making it a valuable biomarker for studying these diseases and exploring potential therapeutic interventions.
With its advanced technology and user-friendly design, the Human PK2 CLIA Kit is an essential tool for researchers looking to investigate the role of prokineticin-2 in health and disease. Order now and take your research to the next level with this innovative assay kit from Assay Genie.
Product Name:
Human PK2 (Prokineticin 2) CLIA Kit
SKU:
HUES01013
Target:
Human PK2 (Prokineticin 2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human PK2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PK2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PK2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human PK2. You can calculate the concentration of Human PK2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-105
91-106
94-105
Average (%)
96
97
99
1:4
Range (%)
93-109
101-119
99-112
Average (%)
99
109
106
1:8
Range (%)
102-118
88-102
98-113
Average (%)
109
94
106
1:16
Range (%)
95-108
98-112
98-115
Average (%)
102
105
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-101
95
EDTA plasma (n=5)
94-107
99
Cell culture media (n=5)
85-97
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
197.95
344.89
1510.81
207.21
359.74
1587.06
Standard deviation
19.32
24.83
119.51
17.32
30.79
116.81
C V (%)
9.76
7.2
7.91
8.36
8.56
7.36
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human PK2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PK2 in samples. No significant cross-reactivity or interference between Human PK2 and analogues was observed.
