The Human PKR2 (Prokineticin Receptor 2) CLIA Kit is a highly sensitive assay designed for the accurate detection of Prokineticin Receptor 2 levels in human biological samples. This kit is specifically optimized for use with serum, plasma, and cell culture supernatants, providing researchers with reliable and reproducible results for a variety of research applications.Prokineticin Receptor 2 is a key protein involved in various physiological processes, including the regulation of circadian rhythms, immune response, and reproductive function.
Dysregulation of PKR2 has been linked to conditions such as inflammatory diseases, sleep disorders, and infertility, making it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions.With its high sensitivity and specificity, the Human PKR2 CLIA Kit offers researchers a powerful tool for investigating the role of Prokineticin Receptor 2 in health and disease, paving the way for advancements in biomedical research and personalized medicine.
Product Name:
Human PKR2 (Prokineticin Receptor 2) CLIA Kit
SKU:
HUES01015
Target:
Human PKR2 (Prokineticin Receptor 2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human PKR2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PKR2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PKR2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human PKR2. You can calculate the concentration of Human PKR2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-112
103-117
100-115
Average (%)
104
109
107
1:4
Range (%)
94-105
89-102
85-98
Average (%)
100
95
92
1:8
Range (%)
88-104
90-105
96-113
Average (%)
95
97
104
1:16
Range (%)
89-101
85-99
97-112
Average (%)
94
90
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
84-100
91
EDTA plasma (n=5)
96-110
102
Cell culture media (n=5)
97-112
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
211.34
605.7
1956.08
197.61
600.14
1932.79
Standard deviation
22.0
70.56
167.05
16.42
61.09
193.67
C V (%)
10.41
11.65
8.54
8.31
10.18
10.02
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human PKR2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PKR2 in samples. No significant cross-reactivity or interference between Human PKR2 and analogues was observed.
