Human PLAP/ALPP (Placental Alkaline Phosphatase) CLIA Kit
The Human Placental Alkaline Phosphatase (PLAP) CLIA Kit is a powerful tool for accurately measuring PLAP levels in biological samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers reliable and consistent results, making it ideal for a wide range of research applications.PLAP is an important enzyme found in the placenta, with elevated levels often seen in pregnancy-related conditions such as preeclampsia and fetal abnormalities.
It is also used as a tumor marker in certain types of cancer, making it a valuable biomarker for monitoring disease progression and treatment efficacy.Whether studying pregnancy-related complications or cancer biology, the Human PLAP CLIA Kit provides researchers with a precise and efficient method for quantifying PLAP levels, aiding in the understanding and management of various health conditions.
Product Name:
Human PLAP/ALPP (Placental Alkaline Phosphatase) CLIA Kit
SKU:
HUES01075
Target:
Human PLAP/ALPP (Placental Alkaline Phosphatase)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human PLAP/ALPP. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PLAP/ALPP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PLAP/ALPP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human PLAP/ALPP. You can calculate the concentration of Human PLAP/ALPP in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-109
92-106
93-107
Average (%)
102
100
100
1:4
Range (%)
103-118
89-101
101-120
Average (%)
110
94
110
1:8
Range (%)
103-116
90-102
87-102
Average (%)
109
95
93
1:16
Range (%)
90-103
101-113
90-105
Average (%)
95
107
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-106
99
EDTA plasma (n=5)
88-104
95
Cell culture media (n=5)
90-104
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
108.69
295.36
710.94
118.51
279.33
692.59
Standard deviation
9.88
26.02
71.09
11.69
24.55
62.06
C V (%)
9.09
8.81
10.0
9.86
8.79
8.96
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human PLAP/ALPP concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PLAP/ALPP in samples. No significant cross-reactivity or interference between Human PLAP/ALPP and analogues was observed.
