Human PLAU/uPA (Urokinase-Type Plasminogen Activator) CLIA Kit
The Human PLAU (UPA) Urokinase-type Plasminogen Activator CLIA Kit is a cutting-edge tool designed for the precise measurement of PLAU levels in human samples such as serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit delivers dependable and reproducible results, making it an invaluable asset for various research purposes.PLAU, also known as Urokinase-type Plasminogen Activator, plays a vital role in processes such as fibrinolysis, cell migration, and tissue remodeling.
Increased PLAU levels have been linked to various diseases including cancer, inflammatory disorders, and cardiovascular diseases. Therefore, detecting and quantifying PLAU levels can provide valuable insights for studying these conditions and developing targeted therapies.Overall, the Human PLAU (UPA) Urokinase-type Plasminogen Activator CLIA Kit is a must-have tool for researchers seeking to explore the role of PLAU in health and disease. Get your kit today and unlock new possibilities in your research endeavors.
Product Name:
Human PLAU/uPA (Urokinase-Type Plasminogen Activator) CLIA Kit
SKU:
HUES00991
Target:
Human PLAU/uPA (Urokinase-Type Plasminogen Activator)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human PLAU/uPA. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PLAU/uPA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PLAU/uPA, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human PLAU/uPA. You can calculate the concentration of Human PLAU/uPA in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-113
90-104
87-100
Average (%)
106
96
93
1:4
Range (%)
93-107
99-116
86-100
Average (%)
101
106
91
1:8
Range (%)
85-100
95-109
95-107
Average (%)
91
100
102
1:16
Range (%)
96-111
91-104
99-115
Average (%)
102
96
107
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
98-113
105
EDTA plasma (n=5)
98-112
104
Cell culture media (n=5)
88-103
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
50.71
153.72
344.7
47.96
144.02
334.79
Standard deviation
4.23
13.6
30.13
6.2
12.8
22.67
C V (%)
8.34
8.85
8.74
12.93
8.89
6.77
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human PLAU/uPA concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PLAU/uPA in samples. No significant cross-reactivity or interference between Human PLAU/uPA and analogues was observed.
