Human pMAPT /pTAU (phosphorylated microtubule-associated protein tau) ELISA kit
The Human pMAPT (pTau, Phosphorylated Microtubule-Associated Protein Tau) ELISA Kit is specifically designed for the accurate measurement of phosphorylated microtubule-associated protein tau levels in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures precise and reliable results, making it suitable for various research purposes.Phosphorylated microtubule-associated protein tau, or pTau, plays a key role in neurodegenerative disorders such as Alzheimer's disease, where abnormal tau phosphorylation leads to the formation of neurofibrillary tangles.
By measuring pTau levels, researchers can gain valuable insights into the progression of these disorders and potentially identify new therapeutic targets.This ELISA kit is a valuable tool for studying the pathophysiology of neurodegenerative diseases and exploring potential treatment options. Its ease of use and efficiency make it an essential component for any laboratory conducting research in this area.
Product Name:
Human pMAPT /pTAU (phosphorylated microtubule-associated protein tau) ELISA kit
SKU:
HUES03407
Target:
Human pMAPT /pTAU (phosphorylated microtubule-associated protein tau) ELISA kit
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human pMAPT/pTAU. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human pMAPT/pTAU and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human pMAPT/pTAU, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human pMAPT/pTAU. You can calculate the concentration of Human pMAPT/pTAU in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-99
89-103
91-102
Average (%)
93
96
97
1:4
Range (%)
92-106
83-96
83-96
Average (%)
100
88
88
1:8
Range (%)
95-108
83-97
88-99
Average (%)
100
88
94
1:16
Range (%)
87-101
82-94
88-101
Average (%)
94
89
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-100
94
EDTA plasma (n=5)
90-104
97
Cell culture media (n=5)
93-105
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
106.18
265.31
823.56
98.61
252.19
764.75
Standard deviation
5.55
15.63
27.42
6.78
11.58
25.93
C V (%)
5.23
5.89
3.33
6.88
4.59
3.39
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human pMAPT/pTAU concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human pMAPT/pTAU in samples. No significant cross-reactivity or interference between Human pMAPT/pTAU and analogues was observed.
