The Human PON1 (Paraoxonase-1) CLIA Kit is specifically designed for the quantitative measurement of PON1 levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring accurate and reliable results for various research applications.PON1 is an important enzyme that plays a key role in protecting against oxidative stress and inflammation. It is involved in the metabolism of various substrates, including pesticides and lipid peroxides, making it a crucial biomarker for studying conditions related to oxidative stress, cardiovascular diseases, and neurodegenerative disorders.
By utilizing the Human PON1 CLIA Kit, researchers can accurately measure PON1 levels in biological samples, helping to advance the understanding of its role in disease pathogenesis and potential therapeutic interventions. Get your kit now at AssayGenie to enhance your research capabilities and achieve impactful results in your studies.
Product Name:
Human PON1 (Paraoxonase 1) CLIA Kit
SKU:
HUES01194
Target:
Human PON1 (Paraoxonase 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human PON1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PON1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PON1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human PON1. You can calculate the concentration of Human PON1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-113
102-116
98-113
Average (%)
104
108
104
1:4
Range (%)
98-113
94-105
86-101
Average (%)
104
99
93
1:8
Range (%)
102-114
87-103
95-110
Average (%)
108
95
102
1:16
Range (%)
97-110
101-115
95-108
Average (%)
103
107
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-114
107
EDTA plasma (n=5)
84-98
91
Cell culture media (n=5)
95-107
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
101.79
198.63
730.05
108.67
207.99
757.05
Standard deviation
8.54
22.45
52.05
12.4
24.15
70.48
C V (%)
8.39
11.3
7.13
11.41
11.61
9.31
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human PON1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PON1 in samples. No significant cross-reactivity or interference between Human PON1 and analogues was observed.
