The Human PON1 (Paraoxonase 1) ELISA Kit is specifically designed for the accurate measurement of paraoxonase 1 levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.Paraoxonase 1 is an important enzyme that plays a key role in protecting against oxidative stress and lipid peroxidation. It is involved in the metabolism of organophosphate compounds and is known to have anti-inflammatory and antioxidant properties.
Studies have shown that paraoxonase 1 levels may be altered in various diseases such as cardiovascular diseases, diabetes, and Alzheimer's disease, making it a valuable biomarker for researchers studying these conditions and exploring potential therapeutic interventions.With its superior performance and user-friendly design, the Human PON1 ELISA Kit from Assay Genie is a valuable tool for researchers looking to accurately measure paraoxonase 1 levels in human samples and advance their understanding of its role in health and disease.
Product Name:
Human PON1 (Paraoxonase 1) ELISA Kit
SKU:
HUES03175
Target:
Human PON1 (Paraoxonase 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PON1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PON1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PON1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human PON1. You can calculate the concentration of Human PON1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-100
93-106
85-101
Average (%)
92
98
92
1:4
Range (%)
95-106
80-91
82-94
Average (%)
100
87
89
1:8
Range (%)
89-103
85-100
88-100
Average (%)
96
91
95
1:16
Range (%)
92-102
82-93
86-97
Average (%)
97
87
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-105
98
EDTA plasma (n=5)
86-99
91
Cell culture media (n=5)
95-108
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.46
1.53
4.72
0.45
1.47
4.63
Standard deviation
0.03
0.06
0.26
0.03
0.06
0.19
C V (%)
6.52
3.92
5.51
6.67
4.08
4.1
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human PON1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PON1 in samples. No significant cross-reactivity or interference between Human PON1 and analogues was observed.
