The Human PR3 (Proteinase 3) CLIA Kit is specifically designed for the precise measurement of PR3 levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results for various research purposes.PR3, also known as Proteinase 3, is a key enzyme involved in the immune response and inflammation.
It plays a crucial role in various autoimmune disorders, such as vasculitis and rheumatoid arthritis. Monitoring PR3 levels can provide valuable insights into disease progression and treatment efficacy, making this kit an indispensable tool for researchers and clinicians in the field of immunology and autoimmune diseases.
Product Name:
Human PR3 (Proteinase 3) CLIA Kit
SKU:
HUES01073
Target:
Human PR3 (Proteinase 3)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human PR3. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PR3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PR3, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human PR3. You can calculate the concentration of Human PR3 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-107
94-109
99-116
Average (%)
99
100
107
1:4
Range (%)
88-102
102-117
90-107
Average (%)
93
110
97
1:8
Range (%)
100-113
90-104
93-104
Average (%)
106
96
98
1:16
Range (%)
96-109
99-113
94-111
Average (%)
101
107
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-107
99
EDTA plasma (n=5)
84-98
90
Cell culture media (n=5)
99-111
105
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
91.6
291.47
856.74
90.22
270.24
870.33
Standard deviation
10.38
31.8
71.71
10.12
30.56
92.69
C V (%)
11.33
10.91
8.37
11.22
11.31
10.65
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human PR3 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PR3 in samples. No significant cross-reactivity or interference between Human PR3 and analogues was observed.
