The Human PRF1 (Perforin-1) ELISA Kit is specifically designed for the quantitative detection of perforin-1 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring accurate and reliable results for a variety of research applications.Perforin-1 is a key protein involved in the immune response, particularly in cytotoxic T lymphocytes and natural killer cells.
It plays a crucial role in the elimination of target cells, making it an important biomarker for studying immune function and disorders such as autoimmune diseases, infections, and cancer.With its advanced technology and user-friendly format, the Human PRF1 ELISA Kit is a valuable tool for researchers looking to explore the role of perforin-1 in health and disease, leading to potential new insights and therapeutic strategies.
Product Name:
Human PRF1 (Perforin 1) ELISA Kit
SKU:
HUES02213
Target:
Human PRF1 (Perforin 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.47 ng/mL
Detection range:
0.78-50 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PRF1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PRF1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PRF1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human PRF1. You can calculate the concentration of Human PRF1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-107
95-110
94-105
Average (%)
102
103
100
1:4
Range (%)
90-104
82-93
86-99
Average (%)
95
88
92
1:8
Range (%)
86-100
87-99
80-93
Average (%)
93
92
87
1:16
Range (%)
91-102
84-100
85-96
Average (%)
97
91
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-101
93
EDTA plasma (n=5)
88-102
95
Cell culture media (n=5)
94-112
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.7
6.61
23.43
2.46
7.19
21.35
Standard deviation
0.18
0.3
0.98
0.17
0.42
1.06
C V (%)
6.67
4.54
4.18
6.91
5.84
4.96
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human PRF1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PRF1 in samples. No significant cross-reactivity or interference between Human PRF1 and analogues was observed.
