Human PRF1 (Perforin 1/Pore-Forming Protein) CLIA Kit
The Human PRF1 (Perforin-1) CLIA Kit is specifically designed for the accurate quantitative detection of PRF1 levels in human serum, plasma, and cell culture supernatants. This high-quality kit offers exceptional sensitivity and specificity, ensuring reliable and consistent results for a variety of research applications.PRF1, also known as Perforin-1, is a pore-forming protein that plays a critical role in the immune response by inducing apoptosis in target cells.
Dysregulation of PRF1 has been linked to various autoimmune diseases, immunodeficiencies, and cancer progression, making it a valuable biomarker for studying these conditions and potential therapeutic targets.Overall, the Human PRF1 (Perforin-1) CLIA Kit provides researchers with a powerful tool to accurately measure PRF1 levels and gain insights into its role in various disease processes.
Product Name:
Human PRF1 (Perforin 1/Pore-Forming Protein) CLIA Kit
SKU:
HUES00657
Target:
Human PRF1 (Perforin 1/Pore-Forming Protein)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human PRF1/PFP. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PRF1/PFP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PRF1/PFP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human PRF1/PFP. You can calculate the concentration of Human PRF1/PFP in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-101
93-106
96-111
Average (%)
93
98
104
1:4
Range (%)
95-111
97-112
88-103
Average (%)
103
103
95
1:8
Range (%)
93-107
86-101
95-111
Average (%)
98
93
101
1:16
Range (%)
98-110
102-116
93-109
Average (%)
104
108
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-109
99
EDTA plasma (n=5)
84-96
91
Cell culture media (n=5)
90-104
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
40.88
69.68
348.8
41.6
68.44
367.31
Standard deviation
3.73
4.91
39.03
4.69
8.12
33.79
C V (%)
9.12
7.05
11.19
11.27
11.86
9.2
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human PRF1/PFP concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PRF1/PFP in samples. No significant cross-reactivity or interference between Human PRF1/PFP and analogues was observed.
