The Human PRL (Prolactin) CLIA Kit is specifically designed for the precise detection of prolactin levels in human samples, including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers dependable and consistent results, making it suitable for a diverse range of research applications.Prolactin is a vital hormone that plays a crucial role in lactation, reproduction, and immune regulation. Abnormal levels of prolactin have been linked to various health conditions, such as infertility, breast cancer, and pituitary disorders.
Therefore, accurate measurement of prolactin levels is essential for understanding these conditions and developing potential diagnostic and therapeutic strategies.Overall, the Human PRL (Prolactin) CLIA Kit offers a reliable and efficient solution for researchers studying prolactin and its role in various physiological and pathological processes.
Product Name:
Human PRL (Prolactin) CLIA Kit
SKU:
HUES00125
Target:
Human PRL (Prolactin)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human PRL. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PRL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PRL, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human PRL. You can calculate the concentration of Human PRL in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-110
101-114
84-100
Average (%)
104
108
91
1:4
Range (%)
90-105
84-96
85-98
Average (%)
97
91
92
1:8
Range (%)
93-110
86-99
102-113
Average (%)
100
91
108
1:16
Range (%)
89-103
92-106
86-97
Average (%)
94
100
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-100
93
EDTA plasma (n=5)
96-109
103
Cell culture media (n=5)
93-110
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.09
2.07
9.8
1.03
2.24
9.75
Standard deviation
0.13
0.23
0.8
0.11
0.26
0.9
C V (%)
11.93
11.11
8.16
10.68
11.61
9.23
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human PRL concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PRL in samples. No significant cross-reactivity or interference between Human PRL and analogues was observed.
