Human Pro-MMP-13 (Pro-Matrix Metalloproteinase 13) CLIA Kit
The Human Pro-MMP-13 (Pro-Matrix Metalloproteinase-13) CLIA Kit is a cutting-edge assay designed for the precise measurement of pro-MMP-13 levels in human biological samples. With exceptional sensitivity and specificity, this kit delivers reliable and consistent results, making it a valuable tool for a variety of research applications.Pro-MMP-13 is a key enzyme involved in extracellular matrix remodeling and tissue regeneration.
It plays a critical role in processes such as wound healing, inflammation, and cartilage degradation, making it a key target for studying conditions like arthritis, fibrosis, and cancer metastasis. The Human Pro-MMP-13 CLIA Kit provides researchers with the ability to accurately quantify pro-MMP-13 levels, allowing for a deeper understanding of its role in disease pathogenesis and potential therapeutic interventions.
Product Name:
Human Pro-MMP-13 (Pro-Matrix Metalloproteinase 13) CLIA Kit
SKU:
HUES00891
Target:
Human Pro-MMP-13 (Pro-Matrix Metalloproteinase 13)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human Pro-MMP-13. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human Pro-MMP-13 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human Pro-MMP-13, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human Pro-MMP-13. You can calculate the concentration of Human Pro-MMP-13 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-105
99-113
99-115
Average (%)
99
106
108
1:4
Range (%)
91-104
87-101
85-99
Average (%)
97
92
92
1:8
Range (%)
97-113
97-113
103-117
Average (%)
105
103
109
1:16
Range (%)
93-107
100-116
92-108
Average (%)
99
109
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-102
94
EDTA plasma (n=5)
100-114
107
Cell culture media (n=5)
92-106
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
50.08
124.35
440.67
47.15
133.29
469.05
Standard deviation
4.07
10.74
39.0
5.74
15.29
45.97
C V (%)
8.13
8.64
8.85
12.17
11.47
9.8
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human Pro-MMP-13 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human Pro-MMP-13 in samples. No significant cross-reactivity or interference between Human Pro-MMP-13 and analogues was observed.
