The Human PROC (Protein C) CLIA Kit is a cutting-edge assay designed for the precise quantification of Protein C levels in human samples, including serum, plasma, and cell culture supernatants. This innovative kit boasts exceptional sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.Protein C is a vital protein in the regulation of blood clotting, acting as an anticoagulant by inhibiting factors involved in the clotting cascade.
Dysregulation of Protein C levels has been linked to various thrombotic disorders and coagulopathies, highlighting its importance as a biomarker in studying these conditions and developing targeted therapies.Overall, the Human PROC (Protein C) CLIA Kit offers unparalleled precision and reliability in monitoring Protein C levels, making it an indispensable tool for researchers and clinicians focused on understanding and treating coagulation disorders.
Product Name:
Human PROC (Protein C) CLIA Kit
SKU:
HUES00675
Target:
Human PROC (Protein C)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human PROC. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PROC and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PROC, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human PROC. You can calculate the concentration of Human PROC in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-104
99-117
99-114
Average (%)
96
106
107
1:4
Range (%)
95-108
99-111
98-115
Average (%)
101
105
105
1:8
Range (%)
90-106
86-101
89-99
Average (%)
97
93
94
1:16
Range (%)
92-106
98-112
85-96
Average (%)
97
103
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-99
93
EDTA plasma (n=5)
89-102
96
Cell culture media (n=5)
89-103
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
53.61
130.21
458.17
49.14
129.61
446.75
Standard deviation
5.5
15.51
29.28
4.98
9.38
36.81
C V (%)
10.26
11.91
6.39
10.13
7.24
8.24
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human PROC concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PROC in samples. No significant cross-reactivity or interference between Human PROC and analogues was observed.
