Human Programmed Cell Death Protein 6 Interacting Protein (PDCD6IP) ELISA Kit
The Human Programmed Cell Death Protein 6 Interacting Protein (PDCD6IP) ELISA Kit is designed for the accurate detection of PDCD6IP levels in human serum, plasma, and cell culture supernatants. This kit features high sensitivity and specificity, ensuring reliable and reproducible results, making it ideal for a wide range of research applications.PDCD6IP, also known as ALIX, is a multifunctional protein that plays a crucial role in regulating programmed cell death, membrane dynamics, and intracellular signaling pathways. It is involved in various cellular processes such as endocytosis, exosome formation, and viral budding, making it a key player in cell biology research and disease mechanisms.
The Human PDCD6IP ELISA Kit provides researchers with a valuable tool to study the functions and interactions of this important protein, offering insights into its role in diseases such as cancer, viral infections, and neurodegenerative disorders. With its high sensitivity and specificity, this kit is essential for advancing our understanding of PDCD6IP biology and developing potential therapeutic interventions.
Product Name:
Human Programmed Cell Death Protein 6 Interacting Protein (PDCD6IP) ELISA Kit
Product Code:
HUDL02224
Size:
96 Assays
Target:
PDCD6IP
Synonyms:
PDCD6-IP, AIP1, Alix, DRIP4, HP95, ALG-2 Interacting Protein X
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-101
91
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
