Human ProGRP (Pro-Gastrin Releasing Peptide) CLIA Kit
The Human ProGRP (Pro-Gastrin Releasing Peptide) CLIA Kit is a highly sensitive and specific assay designed for the precise detection of ProGRP levels in human serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it an invaluable tool for research applications in various fields.ProGRP is a key biomarker associated with neuroendocrine tumors and small cell lung cancer.
It plays a crucial role in regulating cell growth and differentiation, making it a valuable target for studying these diseases and developing potential therapeutic interventions. With its advanced technology and accuracy, the Human ProGRP CLIA Kit is essential for researchers seeking to deepen their understanding of these conditions and explore new treatment strategies.
Product Name:
Human ProGRP (Pro-Gastrin Releasing Peptide) CLIA Kit
SKU:
HUES01100
Target:
Human ProGRP (Pro-Gastrin Releasing Peptide)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ProGRP. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ProGRP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ProGRP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human ProGRP. You can calculate the concentration of Human ProGRP in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-116
91-106
96-111
Average (%)
108
97
103
1:4
Range (%)
89-104
102-116
86-98
Average (%)
96
110
92
1:8
Range (%)
102-113
101-116
86-99
Average (%)
107
108
93
1:16
Range (%)
93-108
91-104
96-112
Average (%)
100
99
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-106
99
EDTA plasma (n=5)
88-98
93
Cell culture media (n=5)
96-110
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
53.31
117.63
490.94
49.31
105.93
493.53
Standard deviation
6.53
12.41
52.19
5.72
9.24
34.4
C V (%)
12.25
10.55
10.63
11.6
8.72
6.97
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human ProGRP concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ProGRP in samples. No significant cross-reactivity or interference between Human ProGRP and analogues was observed.
