Human Proline Rich Protein 4, Lacrimal (PRR4) ELISA Kit
The Human Proline-Rich Protein 4 (Lacrimal PRR4) ELISA Kit is a reliable tool for detecting and quantifying PRR4 levels in human samples. With high sensitivity and specificity, this kit provides accurate and reproducible results, making it ideal for various research applications.PRR4 is a proline-rich protein found in the lacrimal gland, playing a role in maintaining ocular surface health. Abnormal PRR4 levels have been linked to conditions such as dry eye disease and other ocular disorders, making it a valuable biomarker for studying these conditions and developing treatments.
This ELISA kit offers researchers a convenient and efficient way to study PRR4 levels in serum, plasma, and cell culture supernatants, helping to further understand the role of PRR4 in ocular health and disease. Trust in the Human Proline-Rich Protein 4 (Lacrimal PRR4) ELISA Kit for accurate and reliable results in your research endeavors.
Product Name:
Human Proline Rich Protein 4, Lacrimal (PRR4) ELISA Kit
Product Code:
HUDL02415
Size:
96 Assays
Target:
PRR4
Synonyms:
LPRP, PROL4, Lacrimal proline-rich protein, Nasopharyngeal carcinoma-associated proline-rich protein 4
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
