Human Promyelocytic Leukemia Protein (PML) ELISA Kit
The Human Promyelocytic Leukemia Protein (PML) ELISA Kit is specifically designed for the precise and reliable detection of PML levels in human serum, plasma, and cell culture supernatants. This kit is known for its high sensitivity and specificity, ensuring accurate and reproducible results for various research applications.PML is a key protein that plays a critical role in regulating cell growth, apoptosis, and DNA damage response. Dysregulation of PML is associated with various diseases, including leukemia, cancer, and viral infections.
Therefore, the measurement of PML levels can provide valuable insights into disease progression and potential therapeutic targets.With its advanced technology and optimized protocols, the Human PML ELISA Kit from Assay Genie is a valuable tool for researchers studying PML biology and its implications in disease pathogenesis. Get accurate and precise results with this reliable ELISA kit for your research needs.
Product Name:
Human Promyelocytic Leukemia Protein (PML) ELISA Kit
Product Code:
HUDL02356
Size:
96 Assays
Target:
PML
Synonyms:
MYL, RNF71, TRIM19, Probable Transcription Factor PML, Ring Finger Protein 71, Tripartite motif-containing protein 19
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
