The Human PROS (Prothrombin) Protein S CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of PROS levels in human serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it ideal for various research applications.PROS, also known as Prothrombin, is a key protein involved in the blood clotting cascade, playing a crucial role in maintaining hemostasis. Dysregulation of PROS levels can lead to thrombosis or bleeding disorders, making it an important biomarker for studying these conditions and developing potential therapies.
Overall, the Human PROS (Prothrombin) Protein S CLIA Kit offers researchers a convenient and reliable tool for measuring PROS levels, advancing our understanding of hemostasis-related disorders and potential treatment strategies. Visit the URL to learn more about this innovative kit.
Product Name:
Human PROS (Protein S) CLIA Kit
SKU:
HUES00677
Target:
Human PROS (Protein S)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human PROS. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PROS and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PROS, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human PROS. You can calculate the concentration of Human PROS in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-110
92-109
96-108
Average (%)
101
100
102
1:4
Range (%)
100-113
90-101
103-120
Average (%)
107
96
109
1:8
Range (%)
88-100
93-103
94-109
Average (%)
94
98
100
1:16
Range (%)
86-100
89-102
91-103
Average (%)
92
95
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-99
92
EDTA plasma (n=5)
86-100
93
Cell culture media (n=5)
84-98
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
187.85
449.44
1413.49
195.07
475.65
1356.43
Standard deviation
17.51
51.15
152.66
23.72
42.24
134.56
C V (%)
9.32
11.38
10.8
12.16
8.88
9.92
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human PROS concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PROS in samples. No significant cross-reactivity or interference between Human PROS and analogues was observed.
