Human Protein Disulfide Isomerase A2 (PDIA2) ELISA Kit
The Human Protein Disulfide Isomerase A2 (PDIA2) ELISA Kit is specifically designed for the accurate measurement of PDIA2 levels in human samples such as serum, plasma, and cell culture supernatants. This kit provides high sensitivity and specificity, ensuring precise and reliable results for various research applications.PDIA2 is a key protein involved in protein folding, assembly, and degradation, playing a crucial role in maintaining cellular homeostasis. Dysregulation of PDIA2 has been implicated in various diseases, including cancer, neurodegenerative disorders, and autoimmune diseases.
Therefore, the Human PDIA2 ELISA Kit serves as a valuable tool for studying PDIA2's role in disease pathogenesis and developing potential therapeutic interventions.Overall, the Human Protein Disulfide Isomerase A2 (PDIA2) ELISA Kit offers researchers a dependable and efficient method for quantifying PDIA2 levels, providing valuable insights into its biological functions and potential clinical implications.
Product Name:
Human Protein Disulfide Isomerase A2 (PDIA2) ELISA Kit
Product Code:
HUDL02245
Size:
96 Assays
Target:
PDIA2
Synonyms:
PDI-A2, PDA2, PDI, PDIR, PDIP, Protein Disulfide Isomerase,Pancreatic, Protein Disulfide Isomerase-Associated 2, Pancreas-specific protein disulfide isomerase
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
