Human PTGS2/COX-2 (Prostaglandin Endoperoxide Synthase 2) CLIA Kit
The Human PTGS2 (COX-2) Prostaglandin-Endoperoxide Synthase 2 CLIA Kit is a highly sensitive and specific assay designed for the detection of PTGS2 levels in human samples such as serum, plasma, and cell culture supernatants. This kit provides accurate and reproducible results, making it an essential tool for research in various fields.PTGS2, also known as COX-2, is an enzyme involved in the production of prostaglandins, which play crucial roles in inflammation and pain signaling.
Dysregulation of PTGS2 has been linked to various diseases, including cancer, arthritis, and cardiovascular disorders. Therefore, measuring PTGS2 levels can provide valuable insights into disease pathogenesis and therapeutic development.Whether studying the role of PTGS2 in inflammatory pathways or exploring its potential as a therapeutic target, the Human PTGS2 Prostaglandin-Endoperoxide Synthase 2 CLIA Kit offers researchers a reliable and efficient tool for their experiments.
Product Name:
Human PTGS2/COX-2 (Prostaglandin Endoperoxide Synthase 2) CLIA Kit
SKU:
HUES01020
Target:
Human PTGS2/COX-2 (Prostaglandin Endoperoxide Synthase 2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human PTGS2/COX-2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PTGS2/COX-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PTGS2/COX-2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human PTGS2/COX-2. You can calculate the concentration of Human PTGS2/COX-2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-107
102-114
88-101
Average (%)
99
108
96
1:4
Range (%)
99-115
103-119
85-98
Average (%)
105
109
92
1:8
Range (%)
93-105
90-101
102-116
Average (%)
100
96
110
1:16
Range (%)
101-118
99-114
101-116
Average (%)
109
104
107
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
100-116
106
EDTA plasma (n=5)
94-108
102
Cell culture media (n=5)
95-108
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
195.88
335.89
1962.21
183.55
330.82
2095.31
Standard deviation
23.13
36.61
120.48
20.54
30.1
192.14
C V (%)
11.81
10.9
6.14
11.19
9.1
9.17
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human PTGS2/COX-2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PTGS2/COX-2 in samples. No significant cross-reactivity or interference between Human PTGS2/COX-2 and analogues was observed.
