Human PTGS2/COX-2 (Prostaglandin Endoperoxide Synthase 2) ELISA Kit
The Human PTGS2 (COX-2) Prostaglandin-Endoperoxide Synthase 2 ELISA Kit is a powerful tool for accurate quantification of PTGS2 levels in human biological samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring trustworthy and reproducible results for a variety of research applications.PTGS2, also known as COX-2, is an enzyme crucial in the biosynthesis of prostaglandins, playing a vital role in inflammation and pain responses.
Elevated levels of PTGS2 have been linked to various diseases like cancer, cardiovascular issues, and inflammatory disorders. Therefore, the Human PTGS2 ELISA Kit serves as a valuable resource for investigating the role of this enzyme in disease pathogenesis and potential therapeutic interventions.
Product Name:
Human PTGS2/COX-2 (Prostaglandin Endoperoxide Synthase 2) ELISA Kit
SKU:
HUES02800
Target:
Human PTGS2/COX-2 (Prostaglandin Endoperoxide Synthase 2)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PTGS2/COX-2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PTGS2/COX-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PTGS2/COX-2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human PTGS2/COX-2. You can calculate the concentration of Human PTGS2/COX-2 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-109
83-95
87-98
Average (%)
101
90
92
1:4
Range (%)
93-108
86-100
83-93
Average (%)
99
91
88
1:8
Range (%)
93-105
79-94
81-96
Average (%)
98
86
88
1:16
Range (%)
91-104
85-95
85-97
Average (%)
98
90
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-102
94
EDTA plasma (n=5)
95-108
100
Cell culture media (n=5)
86-101
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.98
1.83
7.54
0.93
2.01
7.68
Standard deviation
0.06
0.09
0.32
0.06
0.08
0.38
C V (%)
6.12
4.92
4.24
6.45
3.98
4.95
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human PTGS2/COX-2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PTGS2/COX-2 in samples. No significant cross-reactivity or interference between Human PTGS2/COX-2 and analogues was observed.
