Human Pulmonary Activation Regulated Chemokine (PARC) ELISA Kit
The Human Pulmonary Activation Regulated Chemokine (PARC) ELISA Kit is specifically designed for the accurate quantification of PARC levels in human biological samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures precise and reliable results, making it an ideal tool for a variety of research applications.PARC, also known as CCL18, is a chemokine that plays a key role in the regulation of immune responses and inflammation in the pulmonary system. Elevated levels of PARC have been implicated in various respiratory conditions including asthma, chronic obstructive pulmonary disease (COPD), and pulmonary fibrosis.
Therefore, measuring PARC levels can provide valuable insights into the pathogenesis of these diseases and aid in the development of targeted therapeutic interventions.The Human Pulmonary Activation Regulated Chemokine (PARC) ELISA Kit is a valuable resource for researchers investigating pulmonary diseases and seeking to better understand the mechanisms underlying immune responses in the lungs. Its accuracy, sensitivity, and ease of use make it an indispensable tool for studying the role of PARC in pulmonary inflammation and disease progression.
Product Name:
Human Pulmonary Activation Regulated Chemokine (PARC) ELISA Kit
Product Code:
HUDL02189
Size:
96 Assays
Target:
PARC
Synonyms:
CCL18, AMAC1, SCYA18, DCCK1, MIP-4, CKb7, Alternative Macrophage Activation Associated CC Chemokine 1, Chemokine C-C-Motif Ligand 18, Macrophage inflammatory protein 4
Detection Method:
Sandwich
Range:
78.125-5,000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
