Human TARC (Thymus Activation Regulated Chemokine) ELISA Kit
The Human TARC (Thymus Activation Regulated Chemokine) ELISA Kit is specifically designed for the accurate measurement of TARC levels in human serum, plasma, and cell culture supernatants. This kit is known for its high sensitivity and specificity, ensuring dependable and reproducible results for various research applications.TARC is a chemokine that plays a key role in immune responses and inflammation regulation. It is involved in attracting specific immune cells to sites of inflammation, making it a valuable biomarker for studying inflammatory conditions, allergies, and autoimmune diseases. With the Human TARC ELISA Kit, researchers can effectively measure TARC levels in biological samples, providing insights into disease mechanisms and potential therapeutic targets. Trust in the reliability and precision of this kit for your research needs.
Product Name:
Human TARC (Thymus Activation Regulated Chemokine) ELISA Kit
SKU:
HUES01313
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.