Human RANTES (Regulated On Activation, Normal T-Cell Expressed and Secreted) CLIA Kit
The Human RANTES (Regulated on Activation, Normal T Cell Expressed and Secreted) CLIA Kit is a cutting-edge assay designed for the precise measurement of RANTES levels in human samples such as serum, plasma, and cell culture supernatants. With its superior sensitivity and specificity, this kit delivers accurate and consistent results, making it an invaluable tool for various research applications.RANTES is a key chemokine involved in immune responses, inflammation, and recruitment of immune cells to sites of infection or injury. Dysregulation of RANTES has been linked to various diseases, including autoimmune disorders, inflammatory conditions, and viral infections, highlighting its importance as a biomarker for disease pathology and therapeutic targets.
With the Human RANTES CLIA Kit, researchers can gain valuable insights into the role of RANTES in health and disease, paving the way for innovative diagnostic and therapeutic strategies. Trust in this advanced assay for reliable and robust data that accelerates scientific discovery and clinical advancements.
Product Name:
Human RANTES (Regulated On Activation, Normal T-Cell Expressed and Secreted) CLIA Kit
SKU:
HUES00023
Target:
Human RANTES (Regulated On Activation, Normal T-Cell Expressed and Secreted)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human RANTES. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human RANTES and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human RANTES, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human RANTES. You can calculate the concentration of Human RANTES in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-116
101-117
101-114
Average (%)
106
109
107
1:4
Range (%)
92-104
102-119
86-100
Average (%)
99
109
93
1:8
Range (%)
94-108
93-110
96-112
Average (%)
101
100
103
1:16
Range (%)
98-113
97-114
99-110
Average (%)
106
105
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-103
95
EDTA plasma (n=5)
87-101
92
Cell culture media (n=5)
87-100
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
46.85
110.7
377.51
48.75
113.27
410.75
Standard deviation
5.95
13.15
30.47
5.12
9.8
27.44
C V (%)
12.7
11.88
8.07
10.5
8.65
6.68
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human RANTES concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human RANTES in samples. No significant cross-reactivity or interference between Human RANTES and analogues was observed.
