Human REG1 alpha (Regenerating Islet Derived Protein 1 Alpha) CLIA Kit
The Human REG1 Alpha (Regenerating Islet-Derived Protein 1 Alpha) CLIA Kit is designed for precise measurement of REG1 Alpha levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers excellent sensitivity and specificity, ensuring accurate and consistent results for a variety of research purposes.REG1 Alpha is a key protein known for its role in the regeneration of islet cells and insulin secretion in the pancreas. Dysregulation of REG1 Alpha has been linked to various diseases including diabetes, pancreatitis, and inflammatory conditions.
This makes the REG1 Alpha CLIA Kit a valuable tool for studying these disorders and potentially developing targeted therapies.Overall, the REG1 Alpha CLIA Kit from Assay Genie provides researchers with a reliable and efficient method for quantifying REG1 Alpha levels, further advancing our understanding of its biological functions and implications in human health.
Product Name:
Human REG1 alpha (Regenerating Islet Derived Protein 1 Alpha) CLIA Kit
SKU:
HUES01174
Target:
Human REG1 alpha (Regenerating Islet Derived Protein 1 Alpha)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human REG1α. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human REG1α and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human REG1α, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human REG1α. You can calculate the concentration of Human REG1α in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-114
87-98
103-117
Average (%)
106
93
109
1:4
Range (%)
90-105
95-106
83-98
Average (%)
97
101
90
1:8
Range (%)
85-100
100-114
99-114
Average (%)
91
108
106
1:16
Range (%)
87-99
95-112
89-102
Average (%)
92
103
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
101-115
107
EDTA plasma (n=5)
98-111
104
Cell culture media (n=5)
93-107
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
49.53
145.89
463.35
49.34
151.98
478.89
Standard deviation
5.9
12.62
40.17
4.01
16.78
37.88
C V (%)
11.91
8.65
8.67
8.13
11.04
7.91
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human REG1α concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human REG1α in samples. No significant cross-reactivity or interference between Human REG1α and analogues was observed.
