Human Regenerating Islet Derived Protein 1 Beta (REG1b) ELISA Kit
The Human Regenerating Islet-Derived Protein 1 Beta (REG1B) ELISA Kit is specifically designed for the accurate and sensitive detection of REG1B levels in human serum, plasma, and cell culture supernatants. This kit offers high precision and reliability, making it suitable for a variety of research applications.REG1B, also known as Regenerating Islet-Derived Protein 1 Beta, is a critical factor involved in the regulation of cell growth and regeneration in various tissues, particularly in the pancreas and liver. Dysregulation of REG1B expression has been linked to diseases such as diabetes, liver cirrhosis, and certain types of cancer, highlighting its importance as a potential biomarker for studying these conditions and developing targeted therapies.
With easy-to-follow protocols and excellent performance characteristics, the Human REG1B ELISA Kit from Assay Genie is an essential tool for researchers investigating the role of REG1B in health and disease. Gain valuable insights into the molecular mechanisms underlying various pathologies and contribute to the advancement of personalized medicine with this advanced ELISA kit.
Product Name:
Human Regenerating Islet Derived Protein 1 Beta (REG1b) ELISA Kit
Product Code:
HUDL02518
Size:
96 Assays
Target:
REG1b
Synonyms:
PSPS2, REGH, REGI-BETA, REGL, Pancreatic Stone Protein, Pancreatic Thread Protein, Lithostathine 1 Beta, Secretory Pancreatic Stone Protein 2
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-103
96
Heparin plasma (n=5)
89-102
95
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
