Human REG3gamma (Regenerating Islet Derived Protein 3 Gamma) CLIA Kit
The Human REG3gamma (Regenerating islet-derived protein 3 gamma) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of REG3gamma levels in human serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it suitable for a variety of research applications.REG3gamma is an important protein involved in promoting immune responses and inflammation. It plays a key role in conditions such as inflammatory bowel disease, autoimmune disorders, and bacterial infections.
By measuring REG3gamma levels, researchers can gain valuable insights into these conditions and potentially develop new therapeutic interventions.Overall, the Human REG3gamma CLIA Kit offers researchers a powerful tool for studying the role of REG3gamma in various physiological and pathological processes, advancing our understanding of immune regulation and inflammation.
Product Name:
Human REG3gamma (Regenerating Islet Derived Protein 3 Gamma) CLIA Kit
SKU:
HUES00740
Target:
Human REG3gamma (Regenerating Islet Derived Protein 3 Gamma)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human REG3γ. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human REG3γ and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human REG3γ, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human REG3γ. You can calculate the concentration of Human REG3γ in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-104
101-115
88-101
Average (%)
97
107
93
1:4
Range (%)
86-100
84-96
93-108
Average (%)
93
91
100
1:8
Range (%)
103-116
101-118
95-109
Average (%)
110
108
103
1:16
Range (%)
98-113
92-106
97-112
Average (%)
104
100
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-101
93
EDTA plasma (n=5)
97-111
105
Cell culture media (n=5)
93-104
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.01
2.28
7.09
0.95
2.26
6.6
Standard deviation
0.12
0.19
0.46
0.08
0.2
0.48
C V (%)
11.88
8.33
6.49
8.42
8.85
7.27
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human REG3γ concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human REG3γ in samples. No significant cross-reactivity or interference between Human REG3γ and analogues was observed.
