Human REG3gamma (Regenerating Islet Derived Protein 3 Gamma) ELISA Kit
The Human REG3Gamma (Regenerating Islet-Derived Protein 3 Gamma) ELISA Kit is specifically designed for the precise measurement of REG3Gamma levels in human samples including serum, plasma, and cell culture supernatants. This kit is characterized by its high sensitivity and specificity, ensuring accurate and reproducible results for a variety of research purposes.REG3Gamma is a key protein involved in tissue regeneration and immune response modulation. It is known to play a critical role in various inflammatory conditions, gastrointestinal diseases, and cancer.
By measuring REG3Gamma levels, researchers can gain valuable insights into these diseases and potentially uncover new therapeutic strategies.Overall, the Human REG3Gamma ELISA Kit provides a reliable and efficient tool for studying the role of REG3Gamma in human health and disease, offering researchers a valuable resource for advancing their research in the field.
Product Name:
Human REG3gamma (Regenerating Islet Derived Protein 3 Gamma) ELISA Kit
SKU:
HUES02344
Target:
Human REG3gamma (Regenerating Islet Derived Protein 3 Gamma)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human REG3γ. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human REG3γ and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human REG3γ, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human REG3γ. You can calculate the concentration of Human REG3γ in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-105
94-109
88-99
Average (%)
99
99
93
1:4
Range (%)
89-101
84-95
81-94
Average (%)
95
89
88
1:8
Range (%)
87-101
83-95
86-97
Average (%)
93
89
91
1:16
Range (%)
91-105
88-102
88-100
Average (%)
96
93
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-104
98
EDTA plasma (n=5)
90-100
95
Cell culture media (n=5)
96-112
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
5.16
10.51
38.79
5.19
9.53
38.62
Standard deviation
0.28
0.61
1.65
0.28
0.5
1.56
C V (%)
5.43
5.8
4.25
5.39
5.25
4.04
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human REG3γ concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human REG3γ in samples. No significant cross-reactivity or interference between Human REG3γ and analogues was observed.
